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tra 180  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology tra 180
    Tra 180, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tra+180/pmc12240953-70-18-35?v=Santa+Cruz+Biotechnology
    Average 93 stars, based on 188 article reviews
    tra 180 - by Bioz Stars, 2026-07
    93/100 stars

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    (A) FF-BG02 hESCs maintained with MEF-conditioned medium supplemented with 4 ng/ml bFGF (bar = 100 µm) expressed Oct-4, SSEA-4, and Tra-180. (B) FF-hESCs were then co-cultured with chondrocytes in Transwell tissue culture for 3 weeks. (C) Upon stimulation, fibroblastic cells appeared (as indicated by actin phalloidin staining, red) and stained positive for both Type I collagen and Type II collagen (bar = 20 µm). (D) RT-PCR confirmed that chondrocyte co-culture resulted in upregulation of cartilage-specific markers including aggrecan, Type II collagen, and TGF-β-RII gene expressions and decreased expression of FLK1 and Myf5.

    Journal: PLoS ONE

    Article Title: Derivation of Chondrogenically-Committed Cells from Human Embryonic Cells for Cartilage Tissue Regeneration

    doi: 10.1371/journal.pone.0002498

    Figure Lengend Snippet: (A) FF-BG02 hESCs maintained with MEF-conditioned medium supplemented with 4 ng/ml bFGF (bar = 100 µm) expressed Oct-4, SSEA-4, and Tra-180. (B) FF-hESCs were then co-cultured with chondrocytes in Transwell tissue culture for 3 weeks. (C) Upon stimulation, fibroblastic cells appeared (as indicated by actin phalloidin staining, red) and stained positive for both Type I collagen and Type II collagen (bar = 20 µm). (D) RT-PCR confirmed that chondrocyte co-culture resulted in upregulation of cartilage-specific markers including aggrecan, Type II collagen, and TGF-β-RII gene expressions and decreased expression of FLK1 and Myf5.

    Article Snippet: Cells were blocked with 5% normal goat serum in PBS for 30 minutes, and incubated with rabbit polyclonal antibodies against Types I and II collagen (RDI, Flanders, NJ), Oct-4, SSEA-4, and Tra-180 (Chemicon) with 1∶100 dilutions.

    Techniques: Cell Culture, Staining, Reverse Transcription Polymerase Chain Reaction, Co-Culture Assay, Expressing